Welcome to my Skills & Methods Toolbox — where hands-on lab techniques, scientific know-how, and digital tools converge.
This page maps the practical skills I’ve honed through rigorous training, academic projects, and self-driven learning — the foundation that powers my readiness for real-world challenges in molecular biotech and beyond.

🔬 Laboratory Techniques

Molecular Biology:
PCR (endpoint & genotyping) · DNA/RNA extraction · Plasmid preparation · Molecular cloning (incl. SnapGene in silico design) · Gel electrophoresis · Nucleic acid quantification

Protein Biochemistry:
SDS-PAGE · Western blotting · Protein expression & purification (His-tag / affinity chromatography) · Enzyme assays & kinetics

Cell Culture & Handling:
Mammalian & bacterial cell culture (2D monolayers & 3D organoids) · Routine cell culture techniques (splitting, cryopreservation, cell counting) · Transfection
Immunostaining & Microscopy:
Immunofluorescence (IF) staining · Immunohistochemistry (IHC) · Confocal microscopy

Microbiology:
Bacterial cultivation · Antibiotic resistance testing · Aseptic & sterile techniques

Analytical & Chemical Methods:
Chromatography (HPLC, TLC) · Buffer preparation · pH measurement · Titration

💻 Digital & Data Skills

Data Analysis

  • Excel — lab workflow calculations, reporting, QC (Lab Tools Examples)
  • R / RStudio — ggplot2, dplyr, tidyverse, data wrangling & visualization (Projects Page)
  • Python — BioPython, DNA sequence scripting (Projects Page)
  • ImageJ & Fiji — image analysis
  • LaTeX & Markdown — scientific writing & formatting
  • Git & GitHub — version control & collaboration

Bioinformatics

  • BLAST, sequence alignment, basic primer design

LIMS-style Data Tracking

  • Developed a lightweight metadata tracker in R to organize lab samples and experimental variables (Projects Page)

📚 Scientific Knowledge & Lab Experience

  • Gene expression regulation · Transcription factors · PCR genotyping
  • Autophagy pathways · Nutrient signaling · Starvation models
  • Transgenic animal models (e.g., tf-LC3 mouse for autophagy monitoring)
  • Experimental controls · Protocol troubleshooting
  • Biosafety principles · Sample documentation · Quality standards

🎨 Soft Skills & Communication

  • Fluent in English and German · Native Ukrainian / Russian
  • B2-level Turkish, basic French
  • Strong team collaboration · Excellent communication skills
  • High attention to detail · Analytical mindset · Fast learner
  • Experienced in scientific writing, data presentation, and tutoring