Welcome to my Skills & Methods Toolbox — where hands-on lab techniques, scientific know-how, and digital tools converge.
This page maps the practical skills I’ve honed through rigorous training, academic projects, and self-driven learning — the foundation that powers my readiness for real-world challenges in molecular biotech and beyond.

🔬 Laboratory Techniques

  • Molecular Biology: PCR · DNA/RNA extraction · Plasmid preparation · Cloning (incl. SnapGene in silico) · Gel electrophoresis · Nucleic acid quantification
  • Protein Biochemistry: SDS-PAGE · Western blotting · Protein expression & purification (His-tag / affinity chromatography)
  • Cell Biology: Mammalian & bacterial cell culture · Splitting · Cryopreservation · Cell counting · Immunofluorescence staining · Confocal microscopy · Transfection
  • Microbiology: Cultivation of bacteria · Antibiotic resistance testing · Sterile techniques
  • Chemical Methods: Chromatography (HPLC, TLC) · Titration · Enzyme kinetics & purification · Buffer prep · pH measurement
  • Histological Techniques: ELISA (single and multiplex) · Immunohistochemistry (IHC) · Tissue staining

💻 Digital & Data Skills

  • Data Analysis:
    • Excel (lab workflow calculations · reporting · QC), Lab Tools Examples
    • R / RStudio (ggplot2 · dplyr · tidyverse · data wrangling & visualization), 📁 See Projects Page
    • Python (BioPython · DNA sequence scripting), 📁 See Projects Page
    • ImageJ & Fiji (image analysis)
    • LaTeX & Markdown (scientific writing & formatting)
    • Git & GitHub (version control, collaboration)
  • Bioinformatics:
    • BLAST · Sequence alignment · Primer design basics
  • LIMS-style Data Tracking:
    • Created a lightweight metadata tracker in R to organize lab samples and experimental variables (📁 see Project Page)

📚 Scientific Knowledge & Lab Experience

  • Gene expression regulation · Transcription factors · PCR genotyping
  • Autophagy pathways · Nutrient signaling · Starvation models
  • Transgenic animal models (e.g., tf-LC3 mouse for autophagy monitoring)
  • Familiar with experimental controls · Troubleshooting lab protocols
  • Fluent understanding of biosafety · Sample documentation · Quality standards

🎨 Soft Skills & Communication

  • Fluent in English and German · Native Ukrainian / Russian
  • B2-level Turkish, basic French
  • Strong team collaboration · Excellent communication skills
  • High attention to detail · Analytical mindset · Fast learner
  • Experienced in scientific writing, data presentation, and tutoring